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RMRC No.

RMRC13018

Order

Strain/Stock Designation

FVB/N-Tg(ASS-Ex16-EGFP)16CTssu/Narl

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

Embryos- International: 505USD
Cryo Recovery- International: 690USD

Last Update Date

2016/12/12

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

COI (coisogenic strain),MSR (mutant strain)

Mutant Type

：

TG (Transgenic)

Other Names

：

Background Strain

：

FVB/N

Strain Description

：

1.	The transgene expressed ASS and EGFP as bicistronic transcript. EGFP expression is controlled transcriptionally and posttranscriptionally similar to that of the endogenous ASS gene.
2.	ASS is a rate-limiting enzyme in arginine biosynthesis and play the critical role in the production of nitric oxide. Conceivably, ASS gene expression is complex and dynamic. Particularly the post-transcriptional regulation has been shown to operate in ASS expression. The transgenic mouse system is with modified bacterial artificial chromosome (BAC) carrying ASS gene tagged with coding sequence of EGFP.
3.	The ASS expression profile can be visualized and serves as basis to dissect mechanisms of developmental-specific, tissue-specific ASS regulation. Questions such as physiological response of ASS expression stimulated with a variety of signals can be addressed.
4.	Among lines with EGFP knock-in at exon 16 of ASS gene, Tg(ASS-Ex16-EGFP)16CTsu has about 3 copies of transgene. This line shows relatively high expression in brain which may lead to restless behavior.

Depositor

：

Tsung-Sheng Su

蘇宗笙

：

Allele Symbol	：	Tg(ASS-Ex16-EGFP)16CTssu

Allele Name	：	Transgene insertion 16C, Tsung-Sheng Su

Allele Description	：






Strain of Origin	：	FVB/N

Gene Symbol	：	ASS

Gene Name	：	human argininosuccinate synthetase

Chromosome	：	UN






Genotyping Method	：
Genotyping NLAC	：

Research Application

：

Apoptosis	Cancer
Immunology and Inflammation	Cardiovascular
Cell and Developmental Biology	Metabolism
Neurobiology Research	Diabetes and Obesity

Construction Technologies

：

fluorescent proteins

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x wild type

References

：

PubMed ID: 25616743
PubMed ID: 24884799

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： J Biomed Sci. 2014,13;21:42.; J Biomed Sci. 2015 Jan 23;22(1):10

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： J Biomed Sci. 2014,13;21:42.; J Biomed Sci. 2015 Jan 23;22(1):10

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由Tsung-Sheng Su所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Tsung-Sheng Su

Construct Map

：