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RMRC No. RMRC13385 Order
Strain/Stock Designation C57BL/6-Tg(Miox-NanoLuc)6Narl/Narl
Status
SP| (Cryopreserved or freeze-dried sperm)
Availability Level4
Pricing Academia: 21,527NTD    NSTC: 15,069NTD
Last Update Date 2022/4/18
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： MSR (mutant strain)
Mutant Type ： TG (Transgenic)
Other Names ：
Background Strain ： C57BL/6JNarl
Strain Description ：
1. 
This reporter mouse may serve as an early detection of drug- and food-induced nephrotoxicity and as a mouse model to investigate tubular cell injury, acute kidney injury (AKI) and metabolism.
2.  The BAC-base transgene was designed NanoLuc driven by Miox genes. Miox was selected as the host promoter for renal-specific transgene expression. NanoLuc is inserted into exon 1 of Miox gene and followed after ATG start codon. The transgene was microinjected into C57BL/6J embryos. The founder was crossed with C57BL/6J for germline transmission. The expression levels ofMiox in serum, urine, and kidney were no significant difference between C57BL/6J and heterozygote.
3.  .A strong luminescence intensity was observed in kidney localized in the renal tubule cells, but not in wild type. NanoLuciferase is specifically expressed in the serum, urine, and kidney tissue of this strain.
4.  Luminescence level in blood was significantly increased after 1 day of cisplatin treatment and 3 days of Aristolochic acid (AAI) treatment. The sensitivity is earlier than the test on creatinine and BUN. It can not only reflect nephrotoxicity, but also can be detected before the pathological change of renal tubules damage.
5.  Luminescence produced from Miox-NanoLuc had good stability at room temperature, 4∘C, 20∘C and repeated freeze-thaw cycles.
Depositor ：
Yuan-Yow Chiou / 邱元佑
Email ： yuanyow@mail.ncku.edu.tw
Allele Symbol ： Tg(Miox-NanoLuc)6Narl
Allele Name ： transgenic insertion 6, National Applied Research Laboratories
Allele Description ：
Strain of Origin ： C57BL/6JNarl
Gene Symbol ： Miox
Gene Name ： myo-inositol oxygenase
Chromosome ： UN
Gene Symbol ： NanoLuc
Gene Name ： NanoLuc Luciferase
Chromosome ： UN
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Pharmacology and Toxicology
Construction Technologies ：
luciferase
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x wild type
References ： PubMed ID: 31664498

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: yuanyow@mail.ncku.edu.tw
Construct Map ：
 
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