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RMRC No.

RMRC13365

Order

Strain/Stock Designation

B6.129-Asxl1^tm1Wchou/Narl

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos)　Industry：*　Academia: 7,066NTD　NSTC: 4,923NTD
(Cryo Recovery)　Industry：*　Academia: 12,618NTD　NSTC: 12,618NTD

Last Update Date

2019/8/20

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

MSK (mutant stock),CON (congenic strain)

Mutant Type

：

TM (Target Mutation)

Other Names

：

Background Strain

：

C57BL/6

Strain Description

：

1.	An additional guanine was inserted into this 8-G cassette located at mouse Asxl1 exon 13 to mimic this frequent human ASXL1 mutation. This insertion causes frame shift in the reading frame and introduces a premature stop codon so that the mutant Asxl1 would be shorter than wild type form and lack the c-terminal region which contains a plant homeodomain (PHD).
2.	Mutant Asxl1 expression was driven by the endogenous Asxl1 promoter. Mutant Asxl1 would be expressed identically as the endogenous Asxl1, not restricted to hematopoietic cells.
3.	Both Asxl1 G643WfsX12 heterozygotes and homozygotes are fertile.
4.	Homozygous new-born mice suffered from high rate of post-natal death, with only 7% of viability after weaning. Lack of nursing was probably the main reason of these post-natal lethal events, but the exact causes remains to be elucidated.

Depositor

：

Wen-Chien Chou

周文堅

：

wchou@ntu.edu.tw

Allele Symbol	：	Asxl1^tm1Wchou

Allele Name	：	additional sex combs like 1, target mutation 1, Wen-Chien Chou

Allele Description	：






Strain of Origin	：	129

Gene Symbol	：	Asxl1

Gene Name	：	additional sex combs like 1

Chromosome	：	2






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Hematology	Neurobiology Research
Genetics Research

Construction Technologies

：

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：	No

Colony Maintenance Mating System	：

References

：

PubMed ID: 28697759

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： https://doi.org/10.1186/s13045-017-0508-x

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： https://doi.org/10.1186/s13045-017-0508-x

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由Wen-Chien Chou所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Wen-Chien Chou

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: wchou@ntu.edu.tw

Construct Map

：