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RMRC No.

RMRC13341

Order

Strain/Stock Designation

B6.129-Scube1^tm2Rbya/Narl

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos)　Industry：*　Academia: 7,066NTD　NSTC: 4,923NTD
(Cryo Recovery)　Industry：*　Academia: 12,618NTD　NSTC: 12,618NTD

Last Update Date

2017/10/30

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

MSK (mutant stock),CON (congenic strain)

Mutant Type

：

TM (Target Mutation)

Other Names

：

Background Strain

：

C57BL/6

Strain Description

：

1.	A targeting vector containing aloxP-flanked neomycin-resistant (Neo) cassette inserted the intron 18 of Scube1 and an additional loxP site in the upstream of intron 14 of the gene. Successful targeting of embryonic stem cells with vector above mentioned resulted in a conditional allele including the Neo cassette. Additional Cre-mediated recombination in embryonic stem cells deleted the Neo cassette and produce a conditional allele (Scuble1^Flox) that induce 2 loxP sites flanking exons 15 to 18 which encode a part of the spacer region and the complete 3 cysteine rich (CR) repeat motifs of Scube1. Heterozygous (Scub1^△^/+) mice were generated by crossing male protamine (Prm)-cre; Scuble1^Flox/+ mice to wild-type female mice.
2.	The coding sequence for portion of the spacer region and the 3 CR repeats (exons 15-18) were successfully deleted, yet the COOH-terminal CUB (complement protein C1r/C1s, Uegf, and Bmp1) domain was transcribed via in-frame coding in mutant (△/△) mice platelets. The plasma concentration of SCUBE1 protein was around 150ng/mL in wild-type (+/+), whereas soluble SCUBE1 was undetectable in mutant mice (△/△), and both wild-type and truncated protein could be released from α-granules and tether on the platelet surface upon activation.
3.	This strain showed normal development, survival, and reproduction and no spontaneous bleeding or thrombosis.
4.	Blood cell counts (including platelets) and hematologic and coagulation parameters, as well as protein or mRNA levels of major platelet surface receptors, including glycoprotein VI, glycoprotein IX, glycoprotein Ia (α2), β3 integrin, and ADP receptors (P2Y₁ and P2Y₁₂), were similar between wild-type (+/+) and mutant mice (△/△). In addition, platelet aggregation was indistinguishable in platelet-rich plasma (PRR) samples from wild-type (+/+) and mutant mice (△/△). Furthermore, electron microscopy revealed no apparent differences in ultrastructure or the numbers ofα- or dense granules between wild-type (+/+) and mutant mice (△/△).

Depositor

：

Ruey-Bing Yang

楊瑞彬

：

rbyang@ibms.sinica.edu.tw

Allele Symbol	：	Scube1^tm2Rbya

Allele Name	：	signal peptide, CUB domain, EGF-like 1, target mutation 2, Ruey-Bing Yang

Allele Description	：






Strain of Origin	：	129

Gene Symbol	：	Scube1

Gene Name	：	signal peptide, CUB domain, EGF-like 1

Chromosome	：	15






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Hematology

Immunology and Inflammation

Construction Technologies

：

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x wild type

References

：

PubMed ID: 24833801

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： Wu MY, Lin YC, Liao WJ, Tu CF, Chen MH, Roffler SR, Yang RB. Inhibition of the plasma SCUBE1, a novel platelet adhesive protein, protects mice against

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： Wu MY, Lin YC, Liao WJ, Tu CF, Chen MH, Roffler SR, Yang RB. Inhibition of the plasma SCUBE1, a novel platelet adhesive protein, protects mice against thrombosis. Arterioscler Thromb Vasc Biol. 34: 1390-8, 2014

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由楊瑞彬博士所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Dr. Ruey-Bing Yang

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: rbyang@ibms.sinica.edu.tw

Construct Map

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